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EXPÉRIENCES

EXPÉRIENCES

Five days that quietly rearranged everything

BRIGHTDALE BERLIN, BERLIN, GERMANY  |  MARCH 4-9, 2025
I’ve been trying to figure out how to write this ever since this experience. Every time I sit down, I keep coming back to the same sentence: it’s incredible how much five days can change you.
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Brightdale class of 25’

So let’s start there.

The day is March 3, 2025. I boarded a flight from Seattle to Berlin. On paper, it was a questionable decision at first. My university schedule at first seemingly had barely any give. I had never been to Berlin. I was flying across the world for a startup bootcamp I’d heard about through a LinkedIn connection just a couple months prior. Someone I’d never met in person, in a city I’d never visited, for a program I only half-understood.

I went anyway. Because I’ve learned, slowly and stubbornly, that the moments which look like bad ideas on paper are often the ones that matter most.

The program was Brightdale: a global immersion experience that drops you inside some of the world’s most dynamic tech and startup ecosystems through intensive, boot-camp-style expeditions. Berlin was the first ever stop. I showed up with a carry-on, zero expectations, and a vague sense that something important was about to happen.

I was right.

How a LinkedIn message sent me to Germany

A few months back, I connected with Cesca Centini, Brightdale’s Founder’s Associate, from Italy. It was one of those routine LinkedIn interactions you almost scroll past, but turned into something great after she joined my organization, Youth STEM Initiative, and we started to work on a neuroscience projects + a ton of other stuff (a whole other story). She told me about the Berlin expedition, and something in the way she described it made me stop. Not the usual startup pitch energy. Something more genuine. A real invitation to something real.

Long story short…I told her my schedule was a nightmare and the program date coincides with my final exams that quarter. She encouraged me regardless, connected me with an amazing person called Lucabrando Sanfilippo (Brightdale’s Co-Founder) and left it to me. After much contemplation, a talk with Brando, filling up the application, doing some research, and negotiating with my profs to reschedule exams, here I was on the flight to Germany’s capital on a random Monday in March.

There’s a concept I keep returning to: surface area of luck. The idea is that luck isn’t random. It’s a function of how many interesting situations you put yourself in. Every new experience, every room you walk into, every stranger you say yes to: that’s you expanding that surface area. You can’t control what luck lands on you. You can control how much space you give it to land. That was a life-changing takeaway from this experience.

Brightdale Berlin was me expanding the surface area. And as it turned out, Berlin had A LOT to land.

What actually happened in those five days

I want to be very careful not to over-glamorize this, because the honest truth is that the most memorable parts weren’t the famous logos or the impressive venues that we visited across the city—even though there were plenty of those.

We were guests at Google. We visited a lab in a Berlin suburb doing cutting-edge research on nano enzymes, explained in real time by the scientists actually doing the work. There was a life coaching workshop that hit harder than I expected, the kind of session that quietly rewires how you think about the choices you’ve been putting off. Then at the end of an intense, saturated week, a meditation session that I (and I’m sure everyone else in the cohort) genuinely needed. The week kept giving us things to metabolize, and then gave us the space to metabolize them. That balance wasn’t accidental. It was designed beautifully by Brando, Cesca, and Andrea Parodi, also Brightdale’s Co-Founder.

But the real texture of the week was the founders we met from all the startups and companies we explored in Europe’s San Francisco.

Let me break it down for you in numbers:

19 hrs

with founders across biotech, fintech, VC, healthtech, and edtech

10 hrs

of dinners with real conversations about life, careers, and meaning

2 days

of workshops at Brightdale House in the heart of Berlin

15

incredible friends made from across Europe

Nineteen hours with founders is not a networking event. It’s something closer to being handed a window into how people actually think when they’re building things that don’t exist yet or that they’re ambitiously striving towards making a reality. The mental models. All the failures they talk about so openly. The specific way that someone who has launched three companies and wound down two of them speaks about risk-taking differently from anyone in a classroom ever could.

You start to notice patterns. The best founders weren’t the most confident or cocky people in the room. They were the most curious. They asked more questions than they answered. They were comfortable saying “I don’t know” in a way that felt like a strength rather than a gap. That observation alone has been quietly rearranging the way I carry myself since I came back home.

The dinners

If the daytime sessions were where I learned about the startup world, the dinners were where I learned about everything else.

 

 

 

 

 

 

 

group selfie (Jack Gallazzi, the photographer) at a Vietnamese restaurant

Something happens when you take a group of driven, curious people from across Europe (and America ig), put them around a table with good food and no agenda, and let the conversation go wherever it wants. It goes to the real stuff fast. We talked about what it actually feels like to be in your twenties and unsure of your direction. About how you know when you’re chasing the right thing versus just the impressive-sounding thing. About what happiness looks like when you stop performing it for other people and start actually asking yourself what you truly want in life.

Those weren’t conversations I expected to have at a startup bootcamp. But they were definitely the ones I’ll carry the longest.

The fifteen people I met that week are already the kind of friends where the chats can be active at midnight and the plans to visit each other are already half-formed. Joseph PerrottaLuca coviniEszterIsaac TolleyMarco LomeleGiacomo GallazziKaan DurmazManuel Martín MoranteGabriele SantoroTiberiu-Aurelian TocaFotis TsiroukisKarol Wapniarski—all people from different countries, different fields, different ways of seeing the world, all striving for great things.

That kind of diversity in a room isn’t decorative. It genuinely changes the quality of thought.

The thing Barbara said

“Your unique point of view is your biggest strength.”
— Barbara OrsingherAware

Barbara said this during one of our sessions and I wrote it down immediately. It sounds simple. It isn’t.

We spend so much of our time trying to acquire the “right” knowledge, the “right” skills, the “right” credentials, as if the goal is to make ourselves interchangeable with a better version of everyone else. Sand down the rough edges. Optimize toward some imagined ideal candidate. Get good at the things you’re “supposed to be good at.”

But the things that make you specifically you—your particular combination of experiences, the strange angle you see problems from, the things you find funny, the things that make you unreasonably angry—that’s not noise to be filtered out. That’s the signal. That’s the whole thing. I’ve struggled for a long time to understand this in its full essence and to take it to heart the way I wanted to. But hearing it said out loud in a room full of people who clearly believed it landed differently.

I keep coming back to it.

What I learned

What I’ve written here aren’t polished takeaways. They’re things I wrote in my notes at the end of each day, when the conversations were still fresh in my mind.

  • 1: Take the opportunity without hesitation. The version of you who waits for the perfect moment is protecting something that doesn’t need any protection.

  • 2: The most valuable knowledge doesn’t live in books or on the internet. It lives in people who are in the middle of doing things. Go find them and ask real questions. It can change your life.

  • 3: Building connections is everything. Not in a transactional way, but in the sense that the people you know shape the problems you get to work on and the person you become while working on them.

  • 4: You don’t need to know everything before you start something. You need to find the right people to figure it out with. That’s a completely different problem and a much more solvable one.

  • 5: Consistency will outlast talent, perfectionism, and short-term intensity every. single. time. This in particular is worth repeating to yourself more than once.

  • 6: The people you surround yourself with are the single most important variable. More than your habits, your routine, or your tools. The people.

What Brightdale actually is

There’s a word I keep reaching for and that has inspired me since scrolling through the Brightdale website the first time: agora. The ancient Greek meeting place where ideas collided, where people argued and learned and changed their minds in public, where being part of the conversation was itself the education. Brando, Andrea, and Cesca built something like that. A modern agora, stripped of gatekeeping, designed so that students who wouldn’t get access to these rooms for another decade can walk into them now, early in their exciting careers.

Most of what I’ve gotten from university is theoretical. Knowledge delivered at arm’s length, filtered through (often boring) syllabi and assessment criteria. Brightdale was the opposite of that. It was messy and real and human in a way that lecture halls rarely ever are. I was learning from people who had skin in the game, who had built things and broken things and were in the middle of building again. That context changes everything about how the knowledge lands, what it’s used for.

I’m aware that experiences like this aren’t available to most students globally. The fact that Cesca, Brando, and Andrea have worked together to change that is something I’m genuinely grateful for. Not in the way you say it at the end of a thank you note, but in the way that makes you want to send others in the same direction.

Would I do this again? Don’t even ask.

Berlin · 03.04.25 – 03.09.25

p.s. remember when I said these are the kind of friends where the plans to visit each other are already half-formed? That did indeed come to fruition as I visited Cesca, Jack, Luca, Marco, and Andrea in Milan as well as Kaan and Fotis in Munich the following summer, reflecting on this beautiful experience we had a few months prior and catching up on what life has presented us with ever since.

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Internship at Centre for Cardiovascular Innovation — Centre d’Innovation Cardiovasculaire (CCI-CIC)

VANCOUVER GENERAL HOSPITAL, VANCOUVER, BC, CANADA  |  MARCH 21-24, 2023
During spring break in 2023, I had the splendid opportunity to intern for a short period at the Centre for Cardiovascular Innovation, a clinical research centre operated by the University of British Columbia (UBC) Faculty of Medicine that facilitates regional and international clinical trials for cardiovascular researchers in BC investigating novel therapies for the treatment of certain cardiac conditions. This internship took place at Diamond Health Care Centre and Jim Pattison Pavilion at Vancouver General Hospital in Vancouver, Canada under the guidance of Dr. Brady Robinson. Below are some of the incredible activities that I did at CCI-CIC as well as my reflections on the experience.

                                                  ACTIVITIES

  • Overview of CCI and the innovative projects that CCI researchers are working on to revolutionize cardiac procedures. This was led by Dr. Brady Robinson, Clinical Research Project Manager at CCI-CIC, whose current research project — called COMPLETE TAVR (CTAVR) — focuses on essentially combining Transcatheter Aortic Valve Replacement (TAVR) and Percutaneous Coronary Intervention (PCI) into a single procedure that can help patients with symptomatic aortic stenosis (AS) get treated while managing their concomitant coronary artery disease (CAD). Currently, patients who have CAD and have had PCI done in the past can not receive a TAVR to treat their AS, but CTAVR is set to make it possible to do so. The study is a stratified randomized trial led by the UBC Faculty of Medicine / Vancouver General Hospital with participating medical research institutions across Canada and the United States. 4,000 patients and 100 participating institutions are required for the study (∼200 patients and 60 institutions have been incorporated as of March 2023), and once complete, CTAVR would be the largest study of its kind in the world. Its results will be published in the prestigious New England Journal of Medicine and could have tremendous implications if successful.

  • Observing cardiac catheterization procedures in the Cardiac Catheterization Laboratory (Cath Lab) at Vancouver General Hospital. In these procedures, catheters were inserted through the patients’ common femoral artery and pushed up to the heart to assess blood flow in the coronary arteries with angiography (using X-Rays and contrast dye injected by the catheter). The catheters were also used for PCI: a coronary revascularization procedure performed by the insertion of stents that open up blocked coronary arteries in patients with CAD.

  • Assessing the suitability of several patients to participate in the CTAVR research study using inclusion and exclusion criteria assessing the patients’ current cardiac health (based on measures such as heart rhythm, blood pressure, jet velocity, LVEF, AVA area and index, lesions in coronary arteries, and NYHA Functional Classification during physical activity) and medical history (which entails past cardiac or other related medical conditions and operations).

                                                         REFLECTIONS

For me, the most influential part of my work experience at the UBC Centre for Cardiovascular Innovation was acquiring first-hand insight into COMPLETE TAVR, the flagship research project that the team of CCI researchers is working on. Once again, this developing research study is aiming to develop a method to treat a condition known as aortic stenosis in patients with coronary artery disease using cardiac catheterization, which dismisses the need for surgical procedures. As part of this experience, I got to observe the day-to-day tasks of Dr. Brady Robinson (as a reminder, the Clinical Research Project Manager of CTAVR), who regularly hosted meetings with scientists from different health institutions across Canada and the United States who are participating in the study and sought to recruit new patients for the study based on a set of inclusion/exclusion criteria to determine whether they are suitable to participate in CTAVR. As previously explained, as part of one of my favourite activities, I played the role of a research coordinator as I got to review these criteria and decide whether a set of four patients with different cardiac conditions and medical histories were eligible for the study. And of course, I had the opportunity to observe live cardiac catheterization procedures in the Cath Lab at Vancouver General Hospital, which was a tremendous experience!

During my time at CCI, I learned a lot about how new research studies are formulated and developed through an extensive process of research design development, patient and researcher recruitment, and innovation — one that may take several years to prepare and perform. I also learned how and for what purposes cardiac catheterizations are performed to diagnose and treat cardiovascular conditions like coronary artery disease, as well as how modifications to this cardiac procedure can open up a whole new scope of opportunities to treat cardiovascular diseases without the need for risky surgical operations. After completing this work experience, I have developed an even greater understanding and passion for medical research.

The biggest challenge I had during my placement was probably in the Cath Lab at VGH as I tried to understand step by step what was going on during the cardiac catheterization procedures through the monitors in the control room that displayed the angiographic images and ECG recordings. The angiographic images were black and white and — before the dye was injected by the catheter to highlight the coronary arteries — showed the heart, its arteries and the surrounding parts as faded objects, which sometimes made it difficult to identify where the catheter was during the process and thus what exactly was happening at each stage of the catheterization. The way I overcame this challenge was by simply asking the specialists in the control room to explain to me exactly what I was looking at on the angiogram as well as what was going on at each step, whether it was the insertion of the catheter through the aortic arch and into the heart or the injection of iodine dye into the coronary arteries (a more visually prominent step). Always satisfy your curiosity by asking questions, which in turn will satisfy your thirst for knowledge!

                                                     CONCLUSION & APPRECIATION

My goals for this placement were to deeply explore some of the innovative research taking place at CCI and acquire experiential exposure to the process of research in medicine, which I would love to pursue in the future. I have certainly achieved these goals as I am now much more aware of the large-scale preparation that goes into making studies like COMPLETE TAVR possible.

I sincerely thank Dr. Brady Robinson for spending time off his busy schedule to show me around and teach me about CCI as well as the COMPLETE TAVR project and Dr. Jacqueline Saw for demonstrating first-hand how cardiac catheterizations work in the Cath Lab at VGH. This work experience certainly wouldn’t be so rewarding without them.

 

I laud the efforts being made by researchers at CCI-CIC to innovate in the field of Clinical Cardiology and wish them the greatest success in the future.

Learn more on the CCI-CIC website

TEDxYouth@SunsetBeach

VANCITY THEATRE, VANCOUVER, C.-B., CANADA  |  9 SEPTEMBRE 2022
En janvier 2022, je suis devenu directeur des communications pour un événement TED organisé indépendamment par des jeunes à Vancouver, au Canada, connu sous le nom de TEDxYouth@SunsetBeach. L'événement, qui devait avoir lieu en septembre de la même année, avait pour but de fournir aux étudiants et aux éducateurs de Vancouver et d'ailleurs l'occasion de présenter certains de leurs travaux et expériences incroyables, d'exprimer des opinions sur la façon de résoudre des problèmes urgents et de partager des informations personnelles inspirantes. histoires. De l'amélioration de l'accès à la technologie au Malawi en expédiant des salles de classe portables équipées d'ordinateurs, à un discours influent sur la façon de résoudre la crise des opioïdes au Canada en utilisant les leçons du Portugal, les conférenciers de l'événement nous ont tous franchement impressionnés. 
Faire partie de l'équipe qui a organisé cet incroyable événement TEDx était vraiment spécial. Il y avait beaucoup à faire pour préparer et mettre en scène l'événement, depuis la recherche de sponsors et l'augmentation de la publicité via les médias sociaux jusqu'à l'acquisition d'un lieu et d'une équipe d'ingénieurs de mise en scène. Cependant, tout le travail acharné en valait la peine, car nous avons organisé notre premier événement TED réussi au Vancity Theatre le 9 septembre, accueillant un éventail soigneusement sélectionné de conférenciers de Vancouver et d'autres villes comme Montréal et Chicago. Le thème de l'événement de cette année était "Le monde en mutation" - un thème qui complète le véritable état dans lequel se trouve notre monde dynamique en ce moment à la suite d'événements mondiaux sans précédent, de la pandémie de COVID-19 à l'invasion de l'Ukraine par la Russie._cc781905-5cde -3194-bb3b-136bad5cf58d_

Nous reviendrons l'année prochaine pour notre prochain événement. Pour plus d'informations sur TEDxYouth@SunsetBeach, veuillez visiter notre site Web via le lien suivant :TEDxYouth@SunsetBeach (tedxyouthsunsetbeach.com)

ÉTÉ À HOPKINS 2022 @ UNIVERSITÉ JOHNS HOPKINS

BALTIMORE, MARYLAND, ÉTATS-UNIS  |  11 - 22 JUILLET 2022
Mon séjour à l'Université Johns Hopkins pour le programme pré-universitaire Summer at Hopkins a été, tout bien considéré, une expérience qui a changé ma vie. Non seulement j'ai acquis des connaissances précieuses et des compétences cliniques que j'appliquerais à l'avenir à l'école de médecine et au-delà, mais je me suis également fait des amis pour la vie de partout dans le monde. De plus, j'ai acquis une formidable compréhension de ce qu'est la vie universitaire au cours de mes deux semaines de vie et d'études à Johns Hopkins - et tout ce que je peux dire, c'est que c'est EXCITANT !

En février 2022, j'ai découvert le programme Summer at Hopkins grâce à des recherches individuelles et j'ai été immédiatement fasciné par le catalogue de cours proposés dans le cadre du programme. L'Université Johns Hopkins a toujours été l'une de mes écoles de rêve, j'ai donc vu cela comme une formidable opportunité de découvrir ce que c'est que d'être étudiant à l'université tout en bénéficiant d'une expérience éducative enrichissante. J'ai postulé pour un cours appelé Medical School Intensive, qui était essentiellement une mini simulation de l'école de médecine, et je ne pourrais pas être plus satisfait de ma sélection. 

Grâce au cours intensif de la faculté de médecine, j'ai appris des techniques médicales précieuses telles que la détermination du groupe sanguin, la prise d'une intraveineuse et des procédures chirurgicales de base telles que la suture. De plus, ce qui a rendu le cours vraiment unique, ce sont nos excursions quotidiennes à l'hôpital Johns Hopkins, où nous avons eu le privilège de faire des opérations médicales simulées dans leur centre de simulation médicale. Par exemple, nous avons pu utiliser un mannequin avec un rythme cardiaque simulé pour apprendre à diagnostiquer les maladies cardiaques en écoutant ses différents rythmes cardiaques avec un stéthoscope. Un autre jour au centre de simulation, nous avons appris à effectuer des intubations sur des mannequins enfants et adultes à l'aide de dispositifs médicaux réels. À quelques autres occasions, nous avons eu le privilège de faire des travaux de laboratoire dans les laboratoires d'enseignement de premier cycle ultramodernes de Johns Hopkins en utilisant un équipement de laboratoire auquel nous n'aurions généralement pas accès au lycée.

Parallèlement à ces activités pratiques dans le cadre du cours intensif de la faculté de médecine, nous avons également eu des conférences intéressantes couvrant une grande variété de sujets en médecine, résultant en un équilibre parfait entre théorie et pratique. Les nouveaux concepts et techniques que j'ai appris au cours de mes 2 semaines à Johns Hopkins ont profondément élargi ma perspective du domaine médical et de ce que c'est que de vivre et d'étudier dans l'une des universités les plus prestigieuses au monde. Je recommanderais Summer at Hopkins aux étudiants qui souhaitent participer à une expérience éducative enrichissante au cours de l'été, en particulier s'ils s'intéressent à la médecine. 
Personal Reflections 
“Johns Hopkins University has always been one of my dream schools, so I saw this as a tremendous opportunity to experience what it’s like being a student at the university while benefiting from an enriching educational experience.

In the Medical School Intensive course I learned valuable medical techniques such as blood typing, how to take an IV, and basic surgical procedures such as suturing. In addition, what made the course truly unique was our daily excursions to Johns Hopkins Hospital, where we had the privilege of doing simulated medical operations at the Medicine Simulation Center.

The new concepts and techniques I learned in my 2 weeks at Johns Hopkins profoundly broadened my perspective of the medical field and what it’s like to live and study at one of the world’s most prestigious universities.” -Amirali on his experience with the 2022 Pre-College Course Medical School Intensive

Credit: JHU Summer at Hopkins via Instagram (@jhusummer)

   ÉCOLE D'ÉTÉ DE HARVARD 2022 @ HARVARD UNIVERSITY 

CAMBRIDGE, MASSACHUSETTS, ÉTATS-UNIS  |  25 JUILLET - 5 AOÛT 2022
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Le 23 juillet, le lendemain de la fin de Summer at Hopkins, nous avons parcouru près de 900 km de Baltimore à Boston, en visitant Albany en chemin. Après avoir quitté Baltimore vers midi, nous sommes arrivés à notre hôtel à Boston vers 22 heures. J'ai passé la nuit à faire mes bagages et à me préparer pour le lendemain, au cours duquel j'emménagerais pour ma prochaine grande expérience : la Harvard Summer School. 

Environ 5 mois avant cela, j'ai postulé pour la Harvard Summer School à peu près au même moment que pour Summer at Hopkins. L'application exigeait que mon école envoie chacun de mes relevés de notes de la 8e à la 10e année ainsi qu'une lettre de recommandation de l'un de mes conseillers ou enseignants. De plus, j'ai dû répondre à quatre invites académiques différentes en écrivant un paragraphe pour chacune afin d'exprimer mes réflexions à leur sujet. Dans l'ensemble, le processus de candidature à la Harvard Summer School était plus fastidieux que celui de Summer at Hopkins, et le taux d'acceptation dans le programme était plutôt faible. Compte tenu de la nature compétitive de la candidature, j'ai examiné attentivement et récité chaque paragraphe que j'ai écrit pour m'assurer que mes réponses étaient cohérentes. Enfin, quand tout a été peaufiné, j'ai soumis ma candidature fin février. Après environ quatre semaines, le 29 mars, la décision concernant ma candidature a été rendue et j'ai été informé que j'avais été accepté à la Harvard Summer School. Par coïncidence, j'ai été informé que j'étais entré en été à Hopkins le même jour. J'ai immédiatement accepté leurs offres d'admission et postulé aux deux programmes plus tard dans la journée. Je n'avais jamais prévu que je passerais mon été à Johns Hopkins et à l'Université de Harvard. C'est à ce moment-là que j'ai su que j'allais passer l'été de ma vie. 

Pour la Harvard Summer School, j'ai postulé pour un cours appelé Epigenetics & Gene Regulation. Le cours n'était pas une partie de plaisir. Il s'est étendu sur les principes de la biologie AP pour nous apprendre comment les facteurs environnementaux affectent méticuleusement les mécanismes moléculaires par lesquels nos gènes sont exprimés et codés en protéines en utilisant une terminologie et des explications complexes. La plupart des cours de biologie au lycée et même à l'université se concentrent uniquement sur la génétique et l'hérédité. Cependant, ce cours était unique en ce sens qu'il utilisait les concepts d'expression génique et les combinait avec de nouveaux concepts émergents qui font encore l'objet de recherches et de débats. Cela témoigne du fait que l'épigénétique est un nouveau domaine qui nécessite encore beaucoup d'investigations pour être pleinement compris. Une grande partie des recherches que nous avons examinées dans le cours étaient très récentes et nous ont fourni des informations précieuses sur les études potentielles que nous pourrions étudier à l'avenir pour approfondir la recherche. 

Malgré la difficulté du cours, les encouragements constants et l'excellent enseignement de notre professeur, le Dr Amy Tsurumi, professeur agrégé de chirurgie à la Harvard Medical School, nous ont permis de comprendre plus facilement tout le matériel difficile. Au cours de la première semaine, nous avons appris à travers des conférences quotidiennes toute la théorie sur l'épigénétique et comment l'expression des gènes est régulée par certaines biomolécules en réponse à des stimuli environnementaux. Ensuite, en utilisant nos connaissances, nous avons passé la deuxième semaine du cours à rédiger une proposition de subvention fictive pour les National Institutes of Health (NIH) sur un sujet de choix impliquant un mécanisme épigénétique particulier. Après avoir compilé notre recherche, nous l'avons organisée et structurée dans un document suivant les directives du NIH pour la rédaction d'une proposition de subvention. Enfin, après avoir finalisé nos propositions, nous avons chacun créé une courte présentation de 5 minutes résumant nos recherches et présenté nos travaux le dernier jour du cours. 

 
Ma demande de subvention s'intitulait "L'utilisation d'agents hypométhylants contre l'hyperméthylation de l'ADN dans le carcinome à cellules claires du rein (ccRCC)". La méthylation est le processus d'ajout de groupes méthyle à une molécule particulière. Dans l'ADN, la méthylation est un processus crucial qui aide à réguler l'expression de certains gènes. Cependant, si trop de groupes méthyle sont ajoutés, appelés «hyperméthylation», certaines conditions telles que ccRCC peuvent survenir. En effet, les groupes méthyles en excès empêchent les gènes suppresseurs de tumeurs d'être transcrits par des facteurs de transcription. Lorsque ces gènes suppresseurs de tumeurs ne peuvent pas être transcrits, les protéines suppresseurs de tumeurs ne peuvent pas être synthétisées, ce qui signifie que la formation d'une tumeur potentielle ne peut pas être inhibée. Mes recherches ont essentiellement porté sur les acteurs moléculaires appelés agents hypométhylants qui pénètrent dans le noyau des cellules rénales et déméthylent les gènes suppresseurs de tumeur VHL (von Hippel-Lindau) afin qu'ils puissent être transcrits. Dans ma demande de subvention, j'ai proposé d'utiliser des méthodes de test avancées telles que la RT-qPCR (réaction en chaîne par polymérase quantitative par transcription inverse), MeDIP-Seq (séquençage par immunoprécipitation de l'ADN méthylé) et Western Blotting pour évaluer l'efficacité des agents hypométhylants dans la déméthylation Les gènes VHL ainsi que la quantité d'entre eux seront nécessaires pour assurer un résultat satisfaisant. Avec ces agents hypométhylants, il est possible de prévenir voire potentiellement guérir le ccRCC. 

Une copie de l'article original peut être consultée en cliquant sur le document affiché.
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